Figure 2.

Effect of SeNP injections in vivo on spontaneous ROS production by neutrophils. (А)—The changes in spontaneous ROS production in neutrophils of the control and experimental groups with injection of 0.1 mg Se/kg, 0.5 mg Se/kg, and 2.5 mg Se/kg SeNPs. Data are presented at 7 (blue curve), 14 (red), 21 (green), and 28 days (dark blue) after daily injection. Control is the control group with injection of saline. (В)—Dynamics of changes in spontaneous ROS production in neutrophils of the control (red) and experimental group with intraperitoneal injection of 2.5 mg Se/kg (black) were estimated at 0, 7, 14, 21, and 28 days after daily injection of SeNPs or saline (control). The neutrophil ROS generation was determined by luminol-dependent chemiluminescence with automated multiplate reader (Spark™ 10M multimode microplate reader). Data are shown as the mean of chemiluminescence intensity, arb.units ± SEM of five animals for each group. Statistical analysis of experimental groups versus control was performed with paired t-test. Significance between group means: *** p < 0.001 and ** p < 0.01. Values not marked with an asterisk are n/s—no significance.