Figure 4.

Changes in intracellular expression of neutrophil genes involved in redox homeostasis after administration of 0.1 (A) and 2.5 (B) mg/kg SeNPs for 14 and 28 days. Neutrophils prepared from mice 14 (black bars) and 28 (red bars) days after daily injection of 0.1 mg Se/kg (A) and 2.5 mg Se/kg (B) SeNPs. GAPDH was used as housekeeping gene. Dashed line—level of gene expression in controls (mice with administration of saline). Data are expressed as mean ± SEM of five independent experiments. Statistical analysis of experimental groups versus control was performed with paired t-test and marked with black asterisks. Significance between experimental groups marked with red asterisks: *** p < 0.001, ** p < 0.01, and * p < 0.05; n/s—no significance.