Figure 6.

SeNPs modified unstimulated and stimulated NETs formation in a dose-dependent manner. NETs were released by unstimulated (neutrophils alone) (A) and stimulated (neutrophils + 1 mg/mL zymosan) (B) neutrophils: the control group (saline)—blue, groups with SeNP injection—0.1 mg Se/kg (red), 0.5 mg Se/kg (green), 2.5 mg Se/kg (dark blue). The NETs quantification estimated 0, 7, 14, 21, and 28 days after SeNPs or saline (control) were injected daily. NETs were identified as quantification extracellular DNA of supernatants from neutrophils using fluorometric DNA quantification assay (Pico488 DNA quantification kit) at 503 nm excitation wavelength and at 525 nm detection wavelength. Neutrophils were isolated from eight animals for each experimental group. Data are shown as mean ± SEM. Statistical analysis of experimental groups versus control was performed with paired t-test. Significance between group means: *** p < 0.001 and * p < 0.05.