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. 2022 Nov 1;11(21):3457. doi: 10.3390/cells11213457

Figure 2.

Figure 2

Effects of transfection of placenta-derived exosomes from normal and GDM women with miR-140-3p or miR-574-3p agomir or antagomir on the proliferation, migration, and tube formation of HUVECs in vitro. (A) Verification of placenta-derived exosomal miR-140-3p and miR-574-3p expression by qRT-PCR. EXOGDM transfected with miRNA agomir negative control (miRNA AG NC), miR-140-3p agomir (miR-140-3p AG), or miR-574-3p agomir (miR-574-3p AG). EXONor were transfected with miRNA antagomir negative control (miRNA Anta-AG NC), miR-140-3p antagomir (miR-140-3p Anta-AG), or miR-574-3p antagomir (miR-574-3p Anta-AG). (B) Immunofluorescence staining for placenta-derived exosomes marked with PKH26, (Scale bar: 20 µm). (C) The CCK-8 assay was applied to detect the proliferation of HUVECs after treatment with miRNA AG NC-EXOGDM, miR-140-3p AG-EXOGDM, miR-574-3p AG-EXOGDM, miRNA Anta-AG NC-EXONor, miR-140-3p Anta-AG-EXONor, or miR-574-3p Anta-AG-EXONor. (D) A transwell assay was applied to detect the migration of HUVECs after different treatments, (Scale bar: 20 µm). (E) A tube formation assay was applied to detect the tube formation of HUVECs after different treatments (n = 3, Scale bar: 100 µm). ** p < 0.01. ns: no significance.