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. 2022 Nov 1;11(21):3457. doi: 10.3390/cells11213457

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Placenta-derived exosomal miR-140-3p and miR-574-3p expression was negatively regulated by chemerin. (A) Linear regression analysis of placenta-derived exosomal miR-140-3p expression and chemerin content in placenta tissues in pregnant women (n = 23). (B) Linear regression analysis of placenta-derived exosomal miR-574-3p expression and chemerin content in placenta tissues in pregnant women (n = 23). (C) ChemR23 protein levels in placenta tissues from control mice (Ctrl), chemerin-induced diabetic mice with injection of shRNA-negative control (CHM+sh-NC), and chemerin-induced diabetic mice with injection of chemR23 shRNA (CHM+sh-chemR23) detected by western blotting (n = 3). (D) Relative miR-140-3p and (E) miR-574-3p expression of placenta-derived exosomes from Control (EXO^Ctrl), CHM+sh-NC (EXO^CHM+sh-NC) and CHM+sh-chemR23 (EXO^{CHM+sh-chemR23}) mice measured with qRT-PCR (n = 3). ** p < 0.01.