Production of total IgA in supernatant of organ fragment cultures of PP and small intestine of GF, conventionally reared (CONV), and SFB-monoassociated C3H/HeN mice at various time points. RIA was used to detect IgA. Data are means ± standard errors of the mean. SI, small intestine; D, duodenum; J, jejunum; I, ileum. The number of fragment cultures per tissue per time point ranged from 4 to 14.