Skip to main content
. 2022 Nov 4;11(21):3497. doi: 10.3390/cells11213497

Figure 6.

Figure 6

Has2os was involved in the early repair of muscle injury. (A). Immunohistochemistry results showed the morphology of injured muscle induced by CTX for 3 days. Scale bar = 100 μm. (B). The expression levels of Has2os after muscle injury. (C) The mRNA expression levels of ki67 after muscle injury. (D–F). qRT-PCR assays of the key factors of the JNK/MAPK signaling (MEKK1, MEKK2, MKK4, MKK7, JNK1, JNK2, and c-JUN) after muscle injury. (G). Western blot analysis shows the protein expression of p-JNK and JNK after muscle injury. (H). Relative expression in (G) were calculated. GAPDH was the internal control. The results are normalized to those of with si-NC. Values were presented as means ± SEM. The statistical significance was calculated by t-test.