Table 1.
qPCR primers.
| Gene Name | Primer | Sequence (5′-->3′) |
|---|---|---|
| MyHC | MyHC-RT-qF | CGCAAGAATGTTCTCAGGCT |
| MyHC-RT-qR | GCCAGGTTGACATTGGATTG | |
| Mef2C | Mef2C-RT-qF | ATCCCGATGCAGACGATTCAG |
| Mef2C-RT-qR | AACAGCACACAATCTTTGCCT | |
| MyoD | MyoD-RT-qF | CTACCCAAGGTGGAGATCCTG |
| MyoD-RT-qR | CACTGTAGTAGGCGGTGTCGT | |
| MyoG | MyoG-RT-qF | ATCCAGTACATTGAGCGCCTAC |
| MyoG-RT-qR | GACGTAAGGGAGTGCAGATTGT | |
| Has2os | Has2os-RT-qF | CTCCGGAGCCCCTTTGAC |
| Has2os-RT-qR | ACGCGTTTGGGTGAACTTTG | |
| Has2 | Has2-RT-qF | CATCTGTGGAGATGGTGAAGGTC |
| Has2-RT-qR | AGCCATCCAGTATCTCACGCTG | |
| ki67 | ki67-RT-qF | GAGGAGAAACGCCAACCAAGAG |
| ki67-RT-qR | TTTGTCCTCGGTGGCGTTATCC | |
| Pax7 | Pax7-RT-qF | GTTCGGGAAGAAAGAGGACGAC |
| Pax7-RT-qR | GGTTCTGATTCCACATCTGAGCC | |
| JNK1 | JNK1-RT-qF | CGTTCTCCGCTACGGGCTT |
| JNK1-RT-qR | AGTCTAATGGCTTCATCCAAATA | |
| JNK2 | JNK2-RT-qF | CTGGAGCCCAAGGAATTGT |
| JNK2-RT-qR | GCGTTTGGTTCTGAAAAGGA | |
| MEKK1 | MEKK1-RT-qF | TCCAGAAGTTTGTGTCACGC |
| MEKK1-RT-qR | GGGACACATCTGCTCCTCTT | |
| MEKK2 | MEKK2-RT-qF | CCCAGAGTATGACGACAGTCGA |
| MEKK2-RT-qR | GGTAGACCCTACCAAAAGCTCC | |
| MKK4 | MKK4-RT-qF | TAAACCGTGCTGTCGATTTG |
| MKK4-RT-qR | TCGGACCTCCAGCTTCTG | |
| MKK7 | MKK7-RT-qF | GAAGCAGGAGAACCGTGAGG |
| MKK7-RT-qR | AGGAGCAGGGCTTAGAGTG | |
| C-Jun | C-Jun-RT-qF | GAAAAGTAGCCCCCAACCTC |
| C-Jun-RT-qR | GGGACACAGCTTTCACCCTA | |
| GAPDH | GAPDH-RT-qF | CGTCCCGTAGACAAAATGGT |
| GAPDH-RT-qR | TCAATGAAGGGGTCGTTGAT |