Table 1.
Construction and functional screening of metagenomic libraries to identify degradative enzymes
| Name of libraries | Fosmid backbones | Origins | Number of clones | Size of inserts | Positive clones for sequencing |
|---|---|---|---|---|---|
| ED library | pCT3FK | Elephant feces | 1372 | ~ 40 kb | 3 (2 CMCasesa, 1 xylanase) |
| BM1T library | pCT3FK | Thermophilic digestate, fermenter | 980 | ~ 40 kb | 15 (3 arabinoxylanases, 9 dual activitiesb, 3 xylanases) |
| MOD18 library | pCC1FOS | Thermophilic digestate, fermenter, 50 °C | 20000 | ~ 40 kb | 18 (4 arabinanases, 14 CMCases) |
| T1T2 library | pCC1FOS | Thermophilic digestate, fermenter, 55 °C | 5000 | ~ 40 kb | 19 (2 arabinanases, 2 xylanases, 15 CMCases) |
| Others | pCC1FOS/pCT3FK | various | 5424 | ~ 40 kb | 0 |
| Total | 32776 | 55 |
Positive clones were screened by using chromogenic polysaccharide hydrogel (CPH) substrates and insoluble chromogenic biomass (ICB) substrates
aCMCases: carboxymethyl cellulases
bDual activities: both xylanase and arabinoxylanase activities