Table 2.

Summary of “non-E. coli” contigs from shotgun sequencing of positive fosmid clones

Retrieved non-E. coli contigsa	pCC1FOS fosmid pool (40 clones)	pCT3FK fosmid pool (15 clones)	Both pools
Number of contigs	39	42	81
Number of retrieved contigsb	32	22	54
No. of contigs with CAZymesc	13	18	31
Total length of contigs (bps)	295,166	344,179	639,345
Average length of contigs (bps)d	7568	8194	7893

Positive clones were screened by using chromogenic polysaccharide hydrogel (CPH) substrates and insoluble chromogenic biomass (ICB) substrates

^aContigs from E. coli genomic DNA contamination or from vector backbone were confirmed by the size of contig (far more than 40 kb) and sequence identity searched against NCBI database

^bRetrieved contigs refer to the contigs which could be aligned to at least one Sanger end sequence

^cCAZymes were predicted by dbCAN database

^dAverage length of contigs was calculated by using the sum of the contig lengths divided by the amount of contigs after filtering out the E. coli originated contigs