Table 3.
Statistics of genes for putative fibrolytic enzymes from 55 sequenced fosmids
| CAZy familya | Putative activity b | Gene no. |
|---|---|---|
| Hemicellulose degrading ability (37 putative CAZymes) | ||
| GH10 | Endo-1,4-beta-xylanase [EC:3.2.1.8] | 13 |
| GH2 | β-mannosidase [EC:3.2.1.25] | 4 |
| GH11 | Endo-1,4-beta-xylanase [EC:3.2.1.8] | 4 |
| GH67 | α-Glucuronidase [EC:3.2.1.139] | 1 |
| CE1 | Endo-1,4-beta-xylanase [EC:3.2.1.8] | 2 |
| CE4 | Endo-1,4-beta-xylanase [EC:3.2.1.8] | 3 |
| CE6 | para-Nitrobenzyl esterase [EC:3.1.1.-] | 1 |
| GH4 | α-Galactosidase [EC:3.2.1.22] | 1 |
| GH127 | α-Galactosidase [EC:3.2.1.22] | 1 |
| GH43 | Arabinoxylan arabinofuranohydrolase [EC:3.2.1.55] | 6 |
| GH16 | Endo-1,3–1,4-beta-glycanase ExoK [EC:3.2.1.-] | 1 |
| Cellulose degrading ability (9 putative CAZymes) | ||
| GH3 | β-Glucosidase [EC:3.2.1.21] | 3 |
| GH9 | Endo-glucanase [EC:3.2.1.4] | 3 |
| GH94 | Cellobiose phosphorylase [EC:2.4.1.20] | 1 |
| GH1 | β-Glucosidase [EC:3.2.1.21] | 1 |
| GH8 | Endo-glucanase [EC:3.2.1.4] | 1 |
| Pectic substrate and hemicellulose degrading ability (12 putative CAZymes) | ||
| GH43 | Endo-1,5-alpha-l-arabinosidase [EC:3.2.1.99] | 5 |
| GH51 | α-l-Arabinofuranosidase [EC:3.2.1.55] | 4 |
| GH43 | β-Galactosidase [EC:3.2.1.23] | 2 |
| GH2 | β-Galactosidase [EC:3.2.1.23] | 1 |
| Others | ||
| GH24 | Lysozyme [EC:3.2.1.17] | 1 |
| GH73 | N-acetylmuramoyl-l-alanine amidase [EC:3.5.1.28] | 1 |
| GH13 | Starch synthase (maltosyl-transferring) [EC:2.4.99.16] | 2 |
In total 71 putative CAZyme-encoding genes were annotated by dbCAN database; this table shows the merged results of both dbCAN and KEGG annotations
aThe glycoside hydrolases/carbohydrate esterase families were predicted by use of the dbCAN database;
bEnzyme functions were predicted by use of the Kyoto Encyclopedia of Genes and Genomes (KEGG) database