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. 2022 Oct 27;23(21):13044. doi: 10.3390/ijms232113044

Figure 4.

Figure 4

Figure 4

Figure 4

Principal Component Analysis (PCA) and heat map of genes expressed in hippocampal microvessels of female mice fed with the low glycemic index diet (LGD) and the high glycemic index diet (HGD) in the presence or absence of the soluble epoxide hydrolase inhibitor (sEHI). (A) PCA plot demonstrates the hippocampal microvascular global gene expression profile trends in the low glycemic diet (LGD) and the high glycemic diet (HGD) shown as blue and red circles, respectively, the LGD+sEHI and the HGD+sEHI, shown as purple and green circles, respectively. PCA plot depicts the dataset variance as principal components (PC), and the x and y axes represent the most significant differences. The x axis indicates the percent total variation by PC1, and y-axis indicates the percent total variation by PC2. (B) Heat map showing average signal intensities of genes in rows, and the four different experimental treatment groups in columns, as follows: column 1: low glycemic diet (LGD); column 2: high glycemic diet (HGD); column 3: LGD with soluble epoxide hydrolase inhibitor (sEHI); column 4: HGD+sEHI. Genes with higher means column signal intensity are shown in red and genes with lower means column signal intensity are shown in green. List of genes in this heat map and their mean signal intensities are provided in Supplemental Table S5. (C) Bar graphs show average signal intensities of genes in the LGD (blue), the HGD (red), LGD+sEHI (purple), and the HGD+sEHI (green) groups. Black dots represent log2 signal intensity for each biological replicate, n = 3 mice per group. Rxrg, Pten, Ppp1cc, Ndn, Fabp7, and Atp2a2 genes are significantly altered (p < 0.05) in the LGD+sEHI vs. LGD.