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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: Nat Struct Mol Biol. 2008 Apr 20;15(5):507–514. doi: 10.1038/nsmb.1423

Figure 2.

Figure 2

Burst kinetics of aminoacylation. (a) Pre-steady-state time course of aminoacylation of m1G37-tRNACys (7.5 μM) by SepRS (0.25 μM) in the absence or presence of SepCysS (3.12 μM). (b) Time courses of aminoacylation with increasing concentrations of Sep-tRNACys (0.09–0.9 μM) in pre-steady-state conditions of 7 μM m1G37-tRNACys and 0.5 μM SepRS. (c) Replot of the amplitude of the burst phase vs the concentration of Sep-tRNACys fit to a quadratic equation. The term ± refers to standard deviation.