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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: Nat Struct Mol Biol. 2008 Apr 20;15(5):507–514. doi: 10.1038/nsmb.1423

Figure 4.

Figure 4

The SepRS–SepCysS binary complex. (a) Pull-down of the M. jannaschii SepRS–SepCysS binary complex analyzed by SDS-PAGE and by 12% (w/v)/7M urea PAGE. (b) Pull-down of M. jannaschii EF-1α-GTP and Sep-tRNACys. A control showing EF-1α discrimination against uncharged tRNACys and recognition of Cys-tRNACys is provided in lanes 15 and 16 respectively. (c) Kinetics of the concentration-dependent binding of SepRS to SepCysS monitored by a biosensor. (d) Replot of the response units determined from (c) vs SepRS concentrations fit to a binding equation. (e) Aminoacylation of m1G37-tRNACys by SepRS attached to a metal resin in the absence or presence of SepCysS. The term ± refers to standard deviation.