Table 2.
Single turnover kinetics
| tRNA | M.j SepRS vs. M.j tRNACys(*) | E.coli CysRS vs. E coli tRNACys | ||||
|---|---|---|---|---|---|---|
|
| ||||||
| Kd (μM) |
kchem (s−1) |
kchem/Kd (μM−1s−1) | relative | kchem/Kd (μM−1s−1) | relative | |
|
| ||||||
| WT | 1.7 ± 0.3 | 1.3 ± 0.1 | 0.8 ± 0.1 | 1 | 4.8 | 1 |
| U73G | 0.6 ± 0.1 | 0.04 ± 0.02 | 0.07 ± 0.04 | 0.09 | 1.1× 10−4 | 2 × 10−5 |
| G34C | 1.5 ± 0.3 | 0.048 ± 0.003 | 0.032 ± 0.007 | 0.04 | 0.0041 | 1 × 10−3 |
| C35U | 1.6 ± 0.6 | 0.048 ± 0.008 | 0.03 ± 0.01 | 0.04 | 0.024 | 5 × 10−3 |
| A36G | 3 ± 1 | 0.11 ± 0.03 | 0.04 ± 0.02 | 0.05 | 0.07 | 0.01 |
| G37A | 1.9 ± 0.9 | 0.05 ± 0.01 | 0.03 ± 0.02 | 0.04 | Not determined | |
| A38G | 0.4 ± 0.2 | 0.03 ± 0.004 | 0.08 ± 0.04 | 0.1 | Not determined | |
| G15C-C48G | 0.4 ± 0.2 | 0.13 ± 0.02 | 0.3 ± 0.2 | 0.4 | 0.16 | 0.03 |
*
Transcripts of m1G37-Mj tRNACys were used except G37A, ±: standard deviation.