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. 2022 Oct 31;23(21):13264. doi: 10.3390/ijms232113264

Figure 2.

Figure 2

PAD inhibitor YW3-56 decreases endotoxic mouse death and serum NETs, cfDNA, TNFα, IL-6 and IL-1β levels. (A) In lethal LPS (35 mpk) induced endotoxic stress model, treatment with YW3-56 (10 mpk) decreased LPS mediated mouse death (n = 7 in each group). (B–F) Serum IL-6 levels (n = 6, 7, 6 in each group) (B), TNFα levels (n = 6, 6, 5 in each group) (C), IL-1β levels (n = 4 in each group) (D), NETs levels (n = 8, 9, 9 in each group) (E), and cfDNA levels (n = 10, 11, 9 in each group) (F) were much increased after LPS treatment, while YW3-56 treatment significantly decreased serum IL-6, TNFα, IL-1β, NETs and cfDNA levels. (G) Serum cfDNA levels had a strong correlation with the serum NET levels. The Person’s correlation analysis was used in survival analysis. All data in figures were presented as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. IL, interleukin; TNF, tumor necrosis factor; NET, neutrophil extracellular trap; cfDNA, cell free DNA; LPS, lipopolysaccharides.