Figure 3.

Ouabain accelerated the recovery of potassium currents after trypsinization. (A) The series of representative currents, recorded at different times after trypsinization and re-seeding at confluence. At the top, in red, are shown the series of currents recorded from the cells treated with ouabain. At the bottom, in black, are shown the series of currents without treatment with ouabain. The series shown on the left in blue corresponds to a series of currents recorded from a cell in a mature monolayer, before trypsinization. (B) Graphs (B1–B6) compare the average values (±SE) of the (B1) membrane surface, (B2) τ₀, (B3) τ_v, (B4) density of gmax, (B5) V₅₀ and (B6) Hill’s slope. In all these graphs, the red circles correspond to cells treated with ouabain and the black ones, to untreated cells. The blue lines show the corresponding values obtained from cells before trypsinization. Black asterisks indicate a statistically significant difference between the control and ouabain-treated groups for each time. Blue asterisks denote a statistically significant difference as compared to the mean value before trypsinization (blue lines). The numbers at the top of the graphs indicate the number of repetitions. (*) denotes p < 0.05.