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. 2022 Oct 29;11(21):3420. doi: 10.3390/cells11213420

Figure 1.

Figure 1

Neutrophil and NETs-mediated vessel occlusion in grafts prior to transplant. Morphology was assessed by HPS staining. Vascular occlusion occurs to varying degrees, some arterioles or venules (*) are not occluded (A–C) while others are completely occluded (D). Vascular occlusion mainly originates from neutrophils ((E); MPO; black arrows) and activated neutrophils undergoing NETosis ((F); CitH3; black arrows). Qualitative assessment (G) of vessel occlusion in grafts which led to PGD3 (n = 8) as compared with non-PGD3 (n = 5). Each graft obtained was scored qualitatively for vascular occlusion (0, 25, 50, 75 or 100%). A 100% score means all or most vessels visualized (venules and arterioles) were occluded with neutrophils or NETs, whereas 25/50/75% scores mean about 25/50/75% of the blood vessels were obstructed with neutrophils or NETs. Means and standard deviations are represented with bars and individual scores for each graft are plotted with colored dots (Student t test). Original magnification, ×200.