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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: Epilepsia. 2022 Apr 16;63(7):1835–1848. doi: 10.1111/epi.17248

Fig. 1. Fecal microbial composition before and after LFPI and sham LFPI.

Fig. 1.

(A) Microbial alpha diversity as assessed by the Shannon index of richness and evenness before, 1 week, 1 month, and 7 months following craniotomy in feces from LFPI and sham-LFPI (denoted by “Sh” on the X-axis) rats. P-values were calculated using mixed effects linear models with group, timepoint, and group:timepoint interaction as fixed effects and rat identifier as a random effect. (B) Beta diversity analysis was performed using Bray-Curtis dissimilarity and visualized by principal coordinates (PC1 and PC2) analysis (PCoA). Each dot represents individual sample, differently colored for each timepoint on the left panel, and for each group on the right panel. Symbols in the right panel denote different timepoints. P-values were calculated by repeated measures aware PERMANOVA including group, timepoint, and group:timepoint interaction. (C) PCoA plots showing microbial composition at each of the four timepoints vis-à-vis craniotomy. P-values were calculated by analyzing data at each timepoint separately using PERMANOVA to assess group difference. (D) Phylum and (E) genus level composition in LFPI and sham-LFPI (denoted by S on the X-axis) groups at each timepoint. Shown are detected phyla (D) and the top 16 most abundant genera (E).