Skip to main content
. 2022 Oct 28;23(21):13130. doi: 10.3390/ijms232113130

Figure 4.

Figure 4

BPF inhibits the phosphorylation of NF-κB and MAP kinase in LPS-stimulated macrophages. (A,B) HUVECs were pretreated with BPF (10 μM for 6 h), then LPS (100 ng/mL) was added. Cellular protein was extracted at the indicated times; (C,D) HUVECs were pretreated with the indicated concentrations of BPF for 6 h, then LPS (100 ng/mL) was added. After 1 h, the cellular protein was extracted. (E,F) THP-1-macrophages cells were pretreated with the indicated concentrations of BPF for 6 h, then LPS (100 ng/mL) was added. After 1 h, the cellular protein was extracted (A–C) The levels of the indicated proteins were determined using ELISA. All results are shown as the means ± SD of three different experiments with triple samples. * p < 0.5 compared to LPS only.