TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Known type III effector protein(s) expressed or relevant characteristic(s) | Reference(s) or source |
|---|---|---|
| P. aeruginosa strains | ||
| PA103 | ExoT, ExoU | B. H. Iglewski |
| PA103ΔexoU | ExoT | 4 |
| PA103exoT::Tc | ExoU | 3 |
| PA103ΔexoUexoT::Tc | None | This study |
| PA103toxA::Ω | ExoT, ExoU | B. H. Iglewski |
| PA103exsA::Ω | None | 7 |
| Plasmids | ||
| pMOBexoT::Tc | Allelic replacement vector encoding tetracycline-interrupted exoT | 3, 18 |
| pUCP18 | pUC-derived cloning vector able to replicate in P. aeruginosa | 17 |
| pUCPexoS | Encodes the wild-type ADP-ribosyltransferase ExoS (100% activity) | 12 |
| pUCPexoSE381A | Encodes the noncatalytic mutant ExoSE381A (0.02% activity) | This study and 13 |
| pUCPexoT | Encodes the ADP-ribosyltransferase ExoT (0.2% activity) | 21 |
| pUCPexoY | Encodes the adenylate cyclase ExoY | 23 |
| pUCPexoYK81M | Encodes the noncatalytic mutant ExoYK81M | 23 |