FIG. 3.

β₁-Chain integrins are not required for attachment to intimin. (A) TKO (β₁⁻) or DKO (β₁⁺) cells were preinfected (Preinf.) with EPEC strain JPN15.96/pMAR7 and then dispersed as described in the text. Cells were added to microtiter wells coated with varying concentrations of MBP-Int395. Cell binding was quantitated as described previously (20), and the means of quadruplicate samples are shown. (B) DKO (β₁⁺) or TKO (β₁⁻) cells were mock-infected or infected with EPEC strain JPN15.96/pMAR7 and then added to microtiter wells coated with 5-μg/ml MBP-Int395 or 2-μg/ml MBP-Inv497. EDTA indicates binding in the presence of 10 mM EDTA. Cell binding was quantitated, and the means ± standard deviations of quadruplicate samples are shown. OD₅₇₀, optical density at 570 nm.