Morphology of normal P. persimilis eggs (laid by females interfered with GFP) and unhatched eggs laid by females had each of the five genes interfered. When the mother had PpATPb and PpATPd interfered, all eggs failed to hatch had the developmental basis shriveled. When the mother had PpRpL11 and PpRpS2 interfered, partial eggs had the yolk shriveled, and some eggs just failed to hatch with no obvious morphological change observed. When the mother had Pptra-2 interfered, all eggs produced from the fifth day failed to hatch had the yolk shriveled. Eggs were photographed using the M205 FCA (Leica Microsystems Ltd., Hessian, Germany).