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. 2022 Nov 7;23(21):13625. doi: 10.3390/ijms232113625

Figure 2.

Figure 2

GABA increases DAPI uptake in DI TNC1 astrocytes through Cx43 hemichannels via activation of GABAA receptors. (A) Representative photomicrographs showing DAPI uptake in DI TNC1 astrocytes under normal extracellular Ca2+ conditions (Control), show increased DAPI uptake in response to 1 µM GABA, effect that is prevented by preincubation with 100 µM bicuculline (Bic), 10 nM TAT-Cx43L2 (TAT-L2) or 100 nM BAPTA-AM (BAPTA). Scale bar: 50 µm. (B) Quantification of DAPI uptake. n = 3, with technical triplicates. (C) Concentration response curve for GABA on astroglial DAPI uptake performed to estimate the EC50 for GABA (EC50: 590 ± 30 nM). Error bars: SE; * p ˂ 0.05, ** p ˂ 0.01.