Skip to main content
. 2022 Oct 26;23(21):12983. doi: 10.3390/ijms232112983

Figure 2.

Figure 2

Valdecoxib alleviates the morphological changes to the retina and reduces the number of TUNEL-positive RGCs in retinal ischemia-reperfusion injury. (A) Representative images of vertical sections obtained from retinas in the control, 1d-I/R, 3ds-I/R and 7ds-I/R groups, stained with hematoxylin (blue) and eosin (red). Scale bar = 50 μm. (B) Quantification of the mean total thickness of the retina in the control and 1-, 3- and 7-day post-I/R groups. The retinas of the 3ds-I/R and 7ds-I/R groups were significantly thinner compared to those of the control group. (C) Representative images of vertical sections obtained from retinas in the control, 3ds-I/R, 3ds-I/R+DMSO and 3ds-I/R+VAL groups, stained with hematoxylin (blue) and eosin (red). Scale bar = 50 μm. (D) Images obtained from retinas in the control, I/R, I/R+DMSO and I/R+VAL groups, stained with DAPI (blue), RBPMS (green) and TUNEL (red). Scale bar = 25 μm. RBPMS was used to label RGCs, and TUNEL was used to label apoptotic cells. The data are presented as the mean ± SD of three independent experiments. Each group was composed of five rats. ** p < 0.01, * p < 0.05 vs. sham group.