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. 2022 Oct 26;23(21):12983. doi: 10.3390/ijms232112983

Figure 5.

Figure 5

PERK activator CCT020312 reverses the effect of valdecoxib on the expression of ER stress-related proteins and on the apoptosis-related proteins in the OGD/R model. R28 cells were pretreated with CCT020312 at the concentration of 5 μmol/L for 4 h. (A) Western blot analysis of p-PERK, ATF4, GRP78, CHOP, cleaved caspase 3, bax and bcl-2 levels in the control, OGD/R+DMSO, OGD/R+VAL, OGD/R+VAL+CCT and OGD/R+DMSO+CCT groups. β-tubulin served as the loading control. (B–H) Quantification of expression levels of p-PERK, ATF4, GRP78, CHOP, cleaved caspase 3, bax and bcl-2 in the control, OGD/R+DMSO, OGD/R+VAL, OGD/R+VAL+CCT and OGD/R+DMSO+CCT groups using the densitometric analyses of Western blotting. The bar charts show the quantitative data (normalized by β-tubulin) for each protein relative to the control group (assigned a value of 1). Data are represented as the mean ± SD of three independent experiments. One-way ANOVA is used in B to H. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. OGD/R+DMSO group. $ p < 0.05, $$ p < 0.01, $$$ p < 0.001 vs. OGD/R+VAL group.