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. 1999 Jun;67(6):2692–2699. doi: 10.1128/iai.67.6.2692-2699.1999

FIG. 3.

FIG. 3

Southern blot hybridization of AA plasmids by using AAF/I or AAF/II-expressing cosmid clones as probes. Total plasmid DNA was extracted from the strains indicated below; the DNA was digested with EcoRV and separated by agarose gel electrophoresis. DNA was then blotted and hybridized using cosmid pJPN31 (which expresses AAF/I) (a) or cosmid D6 (which expresses AAF/II) (b). (a) Lane 1, JM221; lane 2, H145-1; lane 3, H194-2. (b) Lane 1, 042; lane 2, Peru49; lane 3, DS244R3; lane 4, 199-1.