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. 2022 Oct 25;27(21):7245. doi: 10.3390/molecules27217245

Figure 1.

Figure 1

DSC extract enriched in ACN reduced 4T1 BC cell viability and ROS production. 4T1 cell viability (A). Cells were treated with ACN (10–80 μg C3G/mL) or WE (160–2500 μg GAE/mL, ~20–320 μg C3G/mL) for 48 h. Cell viability was assessed using the in vitro toxicology resazurin based assay kit. ROS levels (B). Cells were treated with ACN or WE (20–320 μg C3G/mL) for 48 h. Production of ROS was assessed with the Carboxy-H2DFFDA probe. RFU were normalized to cell density assessed using the Janus green reagent as detailed in the materials and methods section. Data are mean ± SEM (n ≥ 6). Significant difference was determined by ANOVA followed by Bonferroni test against untreated control (* p < 0.05, ** p < 0.01, *** p < 0.001).