Figure 2.
Effect of perinatal garlic oil supplementation on (A) plasma level of H2S and (B) thiosulfate, and H2S-producing enzymes in 12-week-old offspring kidneys. (C) Representative Western blots demonstrate cystathionine β-synthase (CBS, ~61 kDa), cystathionine γ-lyase (CSE, ~45 kDa), and 3-mercaptopyruvate sulfurtransferase (3MST, ~52 kDa) bands. The relative protein levels of renal cortical (D) CBS, (E) CSE, and (F) 3MST were calculated. The letters a and b indicate the differences between the groups (p < 0.005, one-way ANOVA); N = 8/group.