Table 1.
Immunohistochemical markers used in lymphoma cases of Tokai University, School of Medicine.
| Marker | Target/Pathway | Primary Antibody | Company |
|---|---|---|---|
| BCL2 | Apoptosis | bcl2/100/D5 | Novocastra |
| BCL6 | Germinal center | LN22 | Novocastra |
| cCASP3 | Apoptosis | Asp175, #9661 | Cell Signaling |
| CASP8 | Apoptosis | active subunit p18, 11B6 | Novocastra |
| CD3 | T lymphocytes | CD3 epsilon, LN10 | Novocastra |
| CD5 | T lymphocytes | 4C7 | Novocastra |
| CD10 | Germinal center | 56C6 | Novocastra |
| CD16 | M1-like macrophages | 2H7 | Novocastra |
| CD20 | B lymphocytes | L26 | Novocastra |
| CD47 | B lymphocytes | D3O7P | Cell Signaling |
| CD68 | Pan-macrophages | 514H12 | Novocastra |
| CD85A/LILRB3 | M2-like macrophages | FRAS92B | CNIO |
| CD163 | M2-like macrophages | 10D6 | Novocastra |
| CDK6 | Cell cycle | 98D | CNIO |
| CSF1 | CSF1R pathway | 2D10 | LSBio |
| CSF1R | M2-like macrophages | 2D10 | LSBio |
| Cyclin D1 | Cell cycle | P2D11F11 | Novocastra |
| E2F1 | Cell cycle | Agro368V | CNIO |
| EBER | Epstein-Barr virus | #PB0589, #AR0833 | Novocastra |
| IKAROS | Cytokine signaling | D6N9Y | Cell Signaling |
| IL10 | M2c-like macrophages | LS-B7432 | Lifespan Bioscience |
| Ki67 | Cell cycle | MM1 | Novocastra |
| LMO2 | Proto-oncogene | 299B | CNIO |
| MARCO | Macrophages | HPA063793 | Atlas antibodies |
| MDM2 | p53 signaling | IF2 | Invitrogen |
| MITF | M2-like macrophages | C5/D5/MAB10775 | Abnova |
| MUM1 | Plasma cells | IRF4, EAU32 | Novocastra |
| MYC | Proto-oncogene | Y69 | Abcam |
| NFKB p105/p50 | NFKB pathway | #3035 | Cell Signaling |
| cPARP | Apoptosis | Asp214, D64E10 | Cell Signaling |
| PD-L1 | Immune checkpoint | E1J2J | Cell Signaling |
| p-p44/42 MAPK | MAPK pathway | Thr202/Tyr204, #4370 | Cell Signaling |
| pSTAT3 | STAT3 pathway | Tyr705, D3A7 | Cell Signaling |
| PTX3 | M2c-like macrophages | PPZ1228 | Perseus Proteomics |
| RGS1 | Signal transduction | Rabbit polyclonal | Thermo Fisher |
| SIRPA | M2-like macrophages | D6I3M | Cell Signaling |
| TNFAIP8 | Apoptosis | #14559-MM01 | Sino Biological |
| TP53 | Cell cycle, apoptosis | DO-7 | Novocastra |
| RGS1 | Signal transduction | Rabbit polyclonal | Thermo Fisher |
CNIO, Centro Nacional de Investigaciones Oncológicas (Spanish National Cancer Research Center).