Figure 2.

Treatment with hGF-CM improves DPSC viability after H₂O₂ exposure. (a–c) DPSC viability exposed to different concentrations (100, 150, and 200 µM) of H₂O₂ for 24 h. (a) Schematic image of the timetable for the viability test depending on the H₂O₂ concentration. (b) Quantified results determined by CCK-8 analysis. (c) Representative live/dead images showing the oxidative stress effects induced by H₂O₂ treatment on DPSCs. (d–f) DPSC viability cultured in either CM, 50% CM, or SFM for another 12 h after exposure to 150 µM H₂O₂ for 12 h (d) Schematic image of the timetable. (e) Cell viability measured by CCK-8 analysis. (f) Representative live/dead images of DPSCs confirmed the antioxidative stress effect of hGF-CM. All data are represented as the mean ± SD. One-way analysis of variance followed (ANOVA) by Tukey’s multiple comparisons test was used to detect statistically significant differences between groups. Represents * p < 0. 05, *** p < 0. 001, **** p < 0.0001, ns = not significant, scale bar = 200 µm. SFM: Serum free medium, GM: Growth medium, CM: Conditioned medium, 50% CM: Conditioned medium diluted by 50% with serum free medium.