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. 2022 Oct 31;23(21):13293. doi: 10.3390/ijms232113293

Figure 4.

Figure 4

Effects of various parameters on thiosulfate (TS) production from 1,4-NQ (NQ) oxidation of 300 μM H2S measured with silver nanoparticles (A–C) or by ion chromatography (D). (A) NQ concentration-dependently increased TS production from 1 to 100 μM and decreased it at 300 μM. (B) Effects of increasing concentrations of NQ with or without 0.1 μM SOD in TS production in 21% or <1% O2. SOD increased TS production at both O2 tensions. Low O2 somewhat decreased TS production with or without TS but did not inhibit it. (C) Effects of 0.1 μM superoxide dismutase (SOD) and 1 μM catalase (Cat), alone or in combination, on TS production from 300 μM H2S in the absence (H2S only) or presence of 10 μM NQ. With H2S alone, TS production was decreased by Cat and slightly increased by SOD + Cat. With H2S plus NQ, TS was greatly increased by SOD, decreased by Cat but unaffected by SOD + Cat. (A–C), Mean + SE, n = 8 wells per treatment. *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared to H2S from same treatment; (B), ###, p < 0.001 compared to response without SOD. (D) Ion chromatography measurements of production of TS (S2O3), sulfite (SO3) and sulfate (SO4) by incubation of 3 mM H2S with 300 μM 1,4-NQ or 2-MNQ. Mean + SE, n = 3 wells per treatment; **, p < 0.01; ***, p < 0.001 compared to H2S only; ##, p < 0.01 compared to 1,4-NQ.