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. 2022 Oct 31;23(21):13293. doi: 10.3390/ijms232113293

Figure 6.

Figure 6

(A) Absorbance spectra of oxidized 1,4-NQ (60 μM, light blue/green lines) and after reduction with either N2 gas (dark blue line) or 1 mM H2S, followed by N2 gas to remove excess H2S (dark green line). (B) Spectra of different concentrations of H2S (as Na2S); (C) spectra of mixed polysulfides (H2Sn) produced by anaerobic incubation of Na2S with powdered sulfur (S8) in 200 mM phosphate buffer (pH 7.4). Numbers indicate approximate wavelength of peaks. (D,E) Absorbance spectra as a function of time of the reaction between 230 μM 1,4-NQ and 115 μM H2S in aerobic (21% O2) and anaerobic (<1% O2) buffer. Left panels (H2S + NQ) of each pair show the full spectra, in the right panels (H2S + NQ) − H2S) the H2S spectrum was subtracted from the H2S + NQ spectrum at each time point. The time, in seconds, after addition of H2S is indicated on the right of panel (E).