Figure 1.

Experimental setup for bulk and single-cell sequencing. For bulk sequencing, RBCs from leukocyte-filtered blood units (n = 4) were purified with density gradient centrifugation and corresponding cell-free supernatant samples were ultracentrifuged to obtain EVs. For single-cell sequencing, a fresh blood sample (n = 1) and a sample from a blood unit (n = 1) were purified with density gradient centrifugation, as well as a fresh blood sample with white blood cell filtering. The total RNA was isolated and used for RNA sequencing.