Table 1.
The most enriched pathways and functions in red blood cell (RBC) and RBC-derived extracellular vesicle (EV) transcriptomes. The top five categories are shown in each group, as well as p-values or p-value ranges.
| RBC Transcriptome | |
|---|---|
| Canonical Pathways | p-value |
| EIF2 Signaling | 2.53 × 10−54 |
| Regulation of eIF4 and p70S6K Signaling | 1.65 × 10−24 |
| Mitochondrial Dysfunction | 1.05 × 10−18 |
| Protein Ubiquitination pathway | 2.77 × 10−17 |
| Oxidative Phosphorylation | 1.78 × 10−16 |
| Molecular and Cellular Functions | p-value range |
| Gene expression | 5.17 × 10−31–2.57 × 10−7 |
| Protein synthesis | 4.81 × 10−30–7.75 × 10−5 |
| Cell Death and Survival | 1.47 × 10−25–1.68 × 10−4 |
| RNA Post-Transcriptional Modification | 7.82 × 10−25–2.78 × 10−5 |
| Cell Cycle | 1.86 × 10−16–1.43 × 10−4 |
| Physiological System Development and Function | p-value range |
| Organismal Survival | 4.93 × 10−14–4.39 × 10−6 |
| Connective Tissue Development and Function | 4.00 × 10−9–5.15 × 10−5 |
| Hematological System Development and Function | 4.25 × 10−9–4.53 × 10−5 |
| Hematopoiesis | 4.25 × 10−9–4.53 × 10−5 |
| Tissue Morphology | 4.25 × 10−9–4.53 × 10−5 |
| EV transcriptome | |
| Canonical Pathways | p-value |
| EIF2 Signaling | 1.14 × 10−64 |
| Oxidative Phosphorylation | 3.21 × 10−25 |
| Mitochondrial Dysfunction | 1.60 × 10−24 |
| Regulation of eIF4 and p70S6K Signaling | 2.90 × 10−18 |
| mTOR Signaling | 1.68 × 10−13 |
| Molecular and Cellular Functions | p-value range |
| Cell Death and Survival | 2.92 × 10−31–4.49 × 10−3 |
| Protein synthesis | 8.21 × 10−24–4.27 × 10−3 |
| Gene expression | 3.53 × 10−19–2.95 × 10−3 |
| RNA Post-Transcriptional Modification | 4.11 × 10−13–7.87 × 10−4 |
| Free Radical Scavenging | 3.24 × 10−9–2.28 × 10−3 |
| Physiological System Development and Function | p-value range |
| Hematological System Development and Function | 6.36 × 10−7–3.56 × 10−3 |
| Connective Tissue Development and Function | 1.10 × 10−6–4.55 × 10−3 |
| Hematopoiesis | 4.02 × 10−6–4.07 × 10−3 |
| Tissue Morphology | 4.02 × 10−6–1.47 × 10−3 |
| Organismal Development | 7.03 × 10−6–2.28 × 10−3 |
Statistically significant overrepresentation of genes was examined on canonical pathways, molecular and cellular functions and physiological system development and function categories with QIAGEN’s Ingenuity Pathway Analysis (Summer, 2018). The significance values are calculated by Fisher’s exact test right tailed.