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. 2022 Nov 5;23(21):13586. doi: 10.3390/ijms232113586

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Inhibition of Gsk-3 activity alters change in global translation under ER stress. Metabolic labelling analysis using AHA in MIN6 cells treated with tunicamycin in the presence or absence of Gsk-3 inhibitor for the indicated time periods. AHA labelled nascent proteins in whole-cell lysates were assessed by Western blotting (a) using HRP-conjugated streptavidin (n = 3). Relative AHA incorporation corrected by α-tubulin was expressed graphically. ** p < 0.01, *** p < 0.001 by two-way ANOVA followed by Bonferroni’s post hoc test. Effect of inhibition of ATF4 function by introducing DN-ATF4 or shRNA(Δ80) on AHA incorporation following (b) a 10 h and (c) a 18 h treatment with tunicamycin (n = 3). Relative AHA incorporation corrected by α-tubulin is shown as mean ± SD in the graph. ** p < 0.01, *** p < 0.01 by one-way ANOVA followed by Bonferroni’s post hoc test. n.s., not significant.