β-Defensin expression in oral tissues by RPA. HBD-1 mRNA, represented by a single protected fragment of 158 bp, was detected in the lateral tongue, gingival tissue, and the parotid gland. In contrast, expression of HBD-2, represented by a single protected fragment of 328 bp, was detectable by RPA only in gingival tissue and was most abundant in gingival tissues associated with inflammation (data not shown).