FIG. 3.

Composite chromatograms of salivary cationic peptides and proteins analyzed by RP-HPLC and CE. (A) RP-HPLC chromatogram, monitored at 206 nm, of peptides eluting with increasing mobile phase (tracing b). The elution positions of purified recombinant HBD-1 and HBD-2 were independently monitored at 206 nm (tracing a). (B) Analysis of HBD-2 immunoreactive fraction 32 (64 min, 53.3% solution B [Fig. 4]) by CE. Absorbance was monitored at 200 nm. The migration times of purified recombinant HBD-1 and HBD-2 were assessed both independent of and in combination with ions present in fraction 32. The lower absorbance trace (tracing b) depicts the ion profile of fraction 32 alone, and the upper trace (tracing a) depicts that of fraction 32 supplemented with HBD-1 and HBD-2 standards. Fraction 32 contains a peptide ion at 22.5 min that has a mobility identical to that of the HBD-2 standard. Lyso, lysozyme.