Table 1.
Immunocytochemical evaluation of AIF (apoptosis inducing factor) in gastric cancer cells after nsPEFs therapy with calcium ions.
| Protocol | EPG85-257P | EPG85-257RDB |
|---|---|---|
| control cells | 100% + (cytoplasm) | 100% ++ (cytoplasm) |
| 12.5 kV/cm | 100% ++ | 100% ++/+++ |
| 25 kV/cm | 100% ++ | 100%, nuclear envelope +; cytoplasm ++ |
| 37.5 kV/cm | 100% ++ | 100% ++/+++ |
| 50 kV/cm | 100% ++ (nuclear envelope) | 100% ++/+++ |
| 1 mM Ca2+ | 100% + | 100% ++ |
| 12.5 kV/cm + 1 mM Ca2+ | 100% ++ | 100% ++/+++ |
| 25 kV/cm + 1 mM Ca2+ | 100% ++/+++ (nuclear loc.) | 100% ++/+++ (nuclear loc.) |
| 37.5 kV/cm + 1 mM Ca2+ | 100% +++ nuclear envelope localization |
98% ++/+++ Nuclear envelope localization |
| 50 kV/cm + 1 mM Ca2+ | 100% +++ reduced cell number | 100% +++ reduced cell number |
| 2 mM Ca2+ | 100% + | 100% +/++ |
| 12.5 kV/cm + 2 mM Ca2+ | 100% + | 100% +/++ |
| 25 kV/cm + 2 mM Ca2+ | 100% ++ (nuclear envelope) | 100% ++/+++ (nuclear envelope) |
| 37.5 kV/cm + 2 mM Ca2+ | 100% ++ (nuclear and cytoplasm) | 100% ++/+++ (nuclear and cytoplasm) |
| 50 kV/cm + 2 mM Ca2+ | 100% +++ damaged cells (nuclei) | 100% +++ damaged cells (nuclear localization) |
The immunohistochemical staining was evaluated as (+) weak, (++) moderate, and (+++) strong.