Figure 4.

(A) Treatment with 15o (2 µM) and 15r (1 µM) in HT-29 cells showed significant G₀/G₁ phase arrest as indicated by the increase in G₀/G₁ phase cell population. (B) Bar graphs depict SubG₁, G₀/G₁, S, and G₂/M cell populations. Statistical significance was determined by two-way ANOVA followed by Tukey’s post-hoc analysis, where *** p < 0.001 represents control vs. 1,2,4-triazolo-linked bis-indolyl (15o and 15r) conjugates in treatment groups and ‘ns’ represents not statistically significant in comparison to control.