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. 2022 Nov 7;27(21):7642. doi: 10.3390/molecules27217642

Figure 8.

Figure 8

Represented images depicting immunofluorescence analysis of (A) NF-κB along with F-Actin in HT-29 cells. Similarly, HT-29 cells were stained with (B) PI3K-p85 and Phalloidin Red. Treatment with different 1,2,4-triazolo-linked bis-indolyl conjugates (15a–r) significantly reduced the expressions of NF-κB in the nucleus and PI3K p85 levels in HT-29 colon cancer cells. Images were acquired using a confocal microscope at 63× and 2× magnification. (C,D) Statistical significance was determined by one-way ANOVA followed by Tukey’s post-hoc analysis, where *** p < 0.001 represents control vs. 1,2,4-triazolo-linked bis-indolyl conjugates (15o and 15r) in the treatment groups.