TABLE 1.
Bleeding times of ACT-treated micea
| Treatment | No. of mice | Mean bleeding time (s) ± SE | Pb |
|---|---|---|---|
| ACT (100 μg/mouse) | 9 | 749 ± 100 | |
| ACTK58Q (100 μg/mouse) | 9 | 242 ± 93 | <0.01 |
| Buffered 8 M urea | 8 | 275 ± 129 | <0.01 |
a
One hundred fifty microliters of toxin solution in 8 M urea containing 20 mM Tris HCl (pH 7.5) and 0.2 mM CaCl2 was intravenously injected to female Slc: ddY mice (8w). Tail tip (2 mm) was then severed under pentobarbital anesthesia, and the tail was immediately immersed in PBS (37°C). Bleeding time was measured from the time of tail transsection to an abrupt stopping of bleeding without rebleeding within 30 s. Bleeding times exceeding 15 min were recorded as 900 s.
b
Student’s t test against ACT-treated group.