Skip to main content
. 2022 Oct 27;23(21):13024. doi: 10.3390/ijms232113024

Figure 1.

Figure 1

Identification of early events in the formation of colitis-associated colonic dysplasia. (A) Treatment regimen used to induce colitis-associated colorectal cancer in Swiss Webster mice. Animals were injected intraperitoneal once with azoxymethane (AOM, 7.4 mg/kg). Each treatment cycle consisted of 7 days of 4% DSS in the drinking water, followed by 14 days of untreated water. Animals were euthanized at the end of the last cycle (day 70). (B) miRs were profiled (FDR 3%) using the nCounter miRNA mouse assay (NanoString®). Red empty boxes—miRs validated by RT-qPCR; (C) Differentially expressed gene targets (n = 97) of the validated miRs that were subjected to in silico analyses (FDR 1%, p < 0.001); (D) Schema of the bioinformatics pipeline for pathway analysis, network interaction, and assessment of the biological impact of the miR signature. Data from in vivo studies (miRs and mRNA global expression) were subjected to bioinformatic analysis.