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. 2022 Oct 27;23(21):13024. doi: 10.3390/ijms232113024

Figure 4.

Figure 4

Impact of transfection with miR-1 on the cell cycle and sub-G1. (A) Effect of the miR-1 mimic vs. the negative control cel-miR-67 (NC) mimic on each phase of the cell cycle, demonstrating the miR-1 mimic increases the percentage of cells in G0/G1. Data are expressed as the mean percentage (± SEM) of cells in each phase and were obtained from 3 experiments. HCT116 and RKO cells were transfected for 48 h and 72 h, respectively. Unpaired t-test with Welch’s correction was used to compare the NC with miR-1 mimics for each phase of the cell cycle, in each cell line; (B) Fold-change of cells in Sub-G1 was calculated by normalizing the percentage of apoptotic cells detected following transfection with the miR-1 mimic, to that of cells transfected with the negative control (cel-miR-67, NC) (mean ± SEM). Comparisons were performed by one sample (or paired) t-test. * p ≤ 0.05; ** p ≤ 0.01.