TABLE 2.
Binding of various peptide concentrations from P. mirabilis, collagen type II, collagen type XI, and negative control to transfected cell lines expressing the HLA class II molecules HLA-DRB1∗0401 and HLA-DRB1∗0402
| Transfectant type | Peptide concn (mg/ml) | Mean fluorescence intensity with:
|
|||
|---|---|---|---|---|---|
| P. mirabilis LEIEKDFTTYGEE | Collagen type II VEIRAEGNRFTY | Collagen type XI DELSPETSPYVKE | Negative control YASGASGASGAS | ||
| HLA-DRB1∗0401 | 0.25 | 1.00 | 1.01 | 1.02 | 1.04 |
| 0.50 | 1.56 | 1.75 | 1.43 | 1.49 | |
| 0.75 | 2.25 | 2.39 | 2.11 | 2.09 | |
| 1.00 | 3.28 | 3.26 | 3.02 | 3.03 | |
| HLA-DRB1∗0402 | 0.25 | 1.00 | 1.01 | 1.00 | 1.02 |
| 0.50 | 1.21 | 1.19 | 1.10 | 1.06 | |
| 0.75 | 1.69 | 1.72 | 1.57 | 1.17 | |
| 1.00 | 1.81 | 1.83 | 1.73 | 1.31 | |