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. 2022 Oct 25;11(21):3365. doi: 10.3390/cells11213365

Figure 2.

Figure 2

Complement inhibitory proteins CD55 and CD59 are higher on Multiple Myeloma peripheral blood EVs relative to healthy control PB EVs. 1.25 × 107 EVs/test from 61 MM patients and 12 healthy controls were bound to the surface of 4 µm aldehyde/sulfate latex beads and stained with antibodies for CD38 and PD-L1 and the complement inhibitory proteins CD55 and CD59 for 30 min on ice. The samples were then resuspended in 200 µL PBS and flow cytometry analysis was performed on a CytoFLEX LX Flow Cytometer. Gating of EV-decorated 4 μm in diameter beads was performed based on FCS/SSC parameters, so that unbound EVs or possible antibody aggregates are excluded from the analysis.