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. 2022 Nov 4;12(21):3040. doi: 10.3390/ani12213040

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Checkerboard titration of capture antibody (cAb) at (a) 2.5 µg/mL, (b) 0.83 µg/mL, (c) 0.28 µg/mL, and (d) 0 µg/mL and titration of detection antibody (dAb) with 10,000, 1000, 100, and 0 pg/mL recombinant IL-2 from left to right represented by . Titration was performed to check for the best combination of antibodies in a broad range of cytokine concentrations.

Inline graphic — Checkerboard titration of capture antibody (cAb) at (a) 2.5 µg/mL, (b) 0.83 µg/mL, (c) 0.28 µg/mL, and (d) 0 µg/mL and titration of detection antibody (dAb) with 10,000, 1000, 100, and 0 pg/mL recombinant IL-2 from left to right represented by . Titration was performed to check for the best combination of antibodies in a broad range of cytokine concentrations.