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. 2022 Nov 4;12(21):3040. doi: 10.3390/ani12213040

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Parallelism of recombinant and native (a) IL-2, (b) IL-6, and (c) IL-12p40 from stimulated PBMCs. As a source of native cytokines, cell culture supernatant of pokeweed mitogen (PWM) stimulated peripheral blood mononuclear cells (PBMCs), isolated from four different chickens, was used. Of these samples, 2× dilutions (chicken 1–4) were tested by capture ELISA and compared with a 2× dilution curve of recombinant cytokines (calibrator). Triangles represent the sample dilutions (and not cytokine concentrations) in comparison to their respective recombinant cytokine (calibration curve). The sample titrations show a high degree of parallelism with the calibration curves of the recombinant cytokines, indicating the similar binding characteristics of the capture ELISAs for the recombinant and native cytokines. Results represent OD_450-620 signal.