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. 2022 Nov 4;12(21):3040. doi: 10.3390/ani12213040

Table 3.

Lower limit of detection (LLOD).

IL-2 IL-6 IL-10 IL-12p40 IFN-γ
LLOD [pg/mL] 12.01 15.11 31.82 3.39 <1.95

The LLOD concentrations were calculated by interpolating from calibration curve the values of the mean OD450-620 + 2 × SD of 24 blank samples containing dilution buffer only.