Table 4.
Parallelism of samples containing native cytokines.
| Parallelism between Recombinant and Native Cytokines [%CV] | |||
|---|---|---|---|
| IL-2 | IL-6 | IL-12p40 | |
| Sample 1 | 7.42 | 9.02 | 12.74 |
| Sample 2 | 15.14 | 6.37 | 11.16 |
| Sample 3 | 10.78 | 7.51 | 15.18 |
| Sample 4 | 7.65 | 6.05 | 14.89 |
Parallelism of the samples was assessed by titrating supernatant of pokeweed mitogen (PWM) stimulated peripheral blood mononuclear cells (PBMCs). Coefficient of variation (%CV) was calculated from samples’ titration that was calculated back according to their dilution factors. All dilutions were run in ELISA in duplicates.